Principal Investigator:

Dr. Piero Crespo, Full Professor CSIC

Other personnel:

• Dra. Lorena Agudo Ibáñez, postdoctoral

• Dr. Javier Rodríguez Martínez, postdoctoral

• Paula Colon Bolea, predoctoral fellow

• Ana Herrero Mier, predoctoral fellow

• José Manuel Gutiérrez Fernandez, predoctoral fellow

• Paula Diez Valle, laboratory technician

Research lines:

Spatial regulation of Ras-ERK signals in physiological processes and in cancer

Funding:

“Las interacciones entre proteínas scaffolds y dímeros de ERK como dianas antitumorales”

BFU2011-23807. 2012-2014.

Relevant publications (last 10 years)

From a total of 80 publications:

1.             Matallanas D., Sanz-Moreno V., Arozarena I., Calvo F., Agudo-Ibáñez L., Santos E., Berciano M.T., and Crespo P. “Distinct utilization of effectors and biological outcomes resulting from site-specific Ras activation: Ras functions in lipid rafts and Golgi complex are dispensable for proliferation and transformation”. Mol. Cell. Biol., 26, 100-116. (2006).

2.             Peregrín S., Jurado M, Sanz-Moreno V, Ruiz-Gómez A, Crespo P., Mayor, jr F. and Murga C. “Phosphorylation of p38 by GRK2 at the docking groove unveils a novel mechanism for inactivating p38 MAPK”.  Curr. Biol., 16, 2042-2047. (2006). (Seleccionado por The Faculty of 1000).

3.             Casar B., Sanz-Moreno V., Yazicioglu M.N., Rodríguez J., Berciano M.T., Lafarga M., Cobb M.H., and Crespo P. “Mxi2 promotes stimulus-independent ERK nuclear translocation”. EMBO J., 26, 635-646. (2007). (Seleccionado por The Faculty of 1000).

4.             Martínez S.E., Lázaro-Diéguez F., Selva J., Calvo F., Renau-Piquéras J., Crespo P., Claro E., and Egea G. “Lysophosphatidic acid rescues Rho A activation and phosphoinositide levels in astrocytes exposed to ethanol”. J. Neurochem., 102, 1044-1052. (2007).

5.             Agudo-Ibáñez L., Núñez F., Calvo F., Berenjeno I.M, Bustelo X.R., and Crespo P. “Transcriptomal profiling of site-specific Ras signals”. Cell. Signal., 19, 2264-2276. (2007).

6.             Vaqué J.P., Fernández-García B., García P., Calvo F., Crespo P, Marín M.C. and León J., “c-Myc inhibits Ras-mediated differentiation of neuroendocrine cells by blocking c-Jun up-regulation”. Mol. Cancer Res., 6, 325-339. (2008).

7.             Casar B., Pinto A., and Crespo P. “Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes”.  Mol. Cell,  31, 708-721. (2008). (Seleccionado por The Faculty of 1000; Editorial en Science Sig. 1, ec315 2008).

8.             González J.M.,  Navarro A., Casar B., Crespo P., and Andrés V. “Fast regulation of AP-1 activity through interaction of lamin A/C,  ERK 1/2 and  c-Fos at the nuclear envelope”. J. Cell Biol., 183, 653-666. (2008). (Seleccionado por The Faculty of 1000).

9.             Casar B., Arozarena I., Sanz-Moreno V., Pinto A., Agudo-Ibáñez L., Marais R., Lewis R.E., Berciano M.T. and Crespo P. “Ras subcellular localization defines ERKs substrate specificity though distinct utilization of scaffold proteins”. Mol. Cell. Biol., 29, 1338-1353. (2009). (Seleccionado por The Faculty of 1000).

10.         Casar B., Pinto A., and Crespo P. “ERK dimers and scaffold proteins: unexpected partners for a forgotten (cytoplasmic) task”.  Cell Cycle, 8, 1007-1013. (2009).

11.         Calvo F., and Crespo P.  “Structural and spatial determinants regulating TC-21 activation by RasGRF family Nucleotide Exchange Factors”. Mol. Biol. Cell, 20, 4289-4302. (2009).

12.         Calvo F., Agudo-Ibáñez L., and Crespo P. “The Ras-ERK pathway: understanding site-specific signaling provides hope of new anti-tumour therapies”. Bioessays. 32, 412-421. (2010).

13.         Pinto A. and Crespo P. “Analysis of ERKs dimerization by electrophoresis”. Meths. Mol. Biol. 661, 335-342. (2010).

14.         Chiariello M., Vaqué J.P., Crespo P., and Gutkind J.S. “Activation of Ras and Rho GTPases and MAP kinases by G-Protein-coupled receptors”. Meths. Mol. Biol. 661, 137-150. (2010).

15.         Rodríguez J., Calvo F., González J.M., Casar B., Andrés V. and Crespo P., “ERK1/2 MAP kinases promote cell cycle entry by rapid, kinase-independent disruption of Retinoblastoma–LaminA complexes”. J. Cell Biol. 191, 967-979. (2010). (Editorial en el mismo número; Editorial en Science Sig. 3, ec371 2010; Seleccionado por The Faculty of 1000).

16.         Matallanas D., and Crespo P. “New druggable targets in the Ras pathway?. Curr. Opin. Mol. Ther. 12, 674-683. (2010).

17.         Arozarena I., Calvo F., Agudo-Ibañez L. and Crespo P. “An actor on many stages: Ras subcellular localization, site-specific regulation and functions”. Genes & Cancer.182-194. (2011).

18.         Calvo F., Sanz-Moreno V, Agudo-Ibáñez L., Wallberg F., Sahai E., Marshall C.J., and Crespo P. “Ras GEFs as inhibitors of Rho GTPases: RasGRF suppresses Cdc42-mediated tumor cell movement, cytoskeletal dynamics and transformation”. Nat. Cell Biol.  13, 819-826. (2011). Seleccionado pro The Faculty of 1000).

19.         Matallanas D., Romano D., O’Neil E., Al-Mulla F., Crespo P., Drosten M., Barbacid M., and Kolch W. “K-Ras oncogene addiction requires the presence of the wildtype allele, EGF signalling and shutdown of MST2 signalling”. Cancer Cell. Contestando críticas de revisores.

 

Contact:

Dr. Piero Crespo

Dpto. Biología Molecular / IBBTEC

Facultad de Medicina, Universidad de Cantabria

(+34) 942 200959

crespop@unican.es